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letter
. 2016 Jan 23;590(2):288–297. doi: 10.1002/1873-3468.12054

Figure 5.

Figure 5

Thermal shift assay. Melt curves to compare the melting temperatures of both apo (A) PBP3 wild‐type and (B) PBP3 S294A with azlocillin and cefoperazone. (A) apo PBP3 blue, T m of 40 ± 1 °C; PBP3 and azlocillin, green, T m of 52 ± 1 °C; PBP3 and cefoperazone, red, T m of 55 ± 1 °C; PBP3 (B) apo PBP3 S294A, blue, T m of 39 ± 1 °C; PBP3 S294A and azlocillin, green, T m of 39 ± 1 °C; PBP3 S294A and cefoperazone, red, T m of 48 ± 1 °C.