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. Author manuscript; available in PMC: 2016 Mar 10.
Published in final edited form as: Nature. 2015 Sep 2;525(7568):256–260. doi: 10.1038/nature14897

Extended data figure 9. Snail and Slug are differentially employed by normal MaSCs and breast TICs.

Extended data figure 9

(a) Kaplan-Meier plots showing survival of patients with the indicated subtypes of breast cancers. Patient groups were separated based on SLUG (top row) or SNAIL (bottom row) mRNA expression. (b) Western blot confirming Slug and Snail knockdown in established PyMT tumour cell line transduced with the indicated shRNA expression vectors. The shLuciferase (shLuc) shRNA was used as a control. (c) Western blot confirming SLUG and SNAIL knockdown in MDA-MB-231 cells transduced with the indicated shRNA expression vectors. shLuc was used as a control. Uncropped western blots are enclosed in Supplemental Information. (d) Tumour-sphere formation efficiencies (# tumourspheres/1000 cells for MDA-MB-361 cells, and # tumourspheres/200 cells for all the other cell lines) of the indicated human breast cancer cells transduced with shSLUG#2, shSNAIL#2, and the shLuc control (mean + s.d., n = 5 technical replicates/group). Data represent two independent experiments. (e, f) SUM159 (e) and SUM149 (f) cells transduced with the indicated shRNAs were injected subcutaneously at limiting dilutions to score primary tumour formation. Tumour-initiation were scored and presented as (# of tumour incidences/# of injections). Data represent two independent experiments. (g) The organoid forming efficiencies of normal MECs transduced with the indicated shRNA expression vectors (mean ± s.d., n =6 technical replicates/group, *p<0.001, N.S. not significant.). Scale bar 100 μm. Data represent three independent experiments.