Table 3. Sensitivity and detection time of the RT-LAMP assay.
| Primer | RNA(copies/test) a | Turbidity detection (LA-200) | Fluorescence detection (Genie III) | ||
|---|---|---|---|---|---|
| Posb | Tp (min) | Posb | Tp (min) | ||
| Trailer | 6800 | 6/6 | 27.0 ± 1.0 | 6/6 | 11.7 ± 1.6 |
| 680 | 6/6 | 40.8 ± 5.9 | 5/6 | 18.3 ± 5.9 | |
| 68 | 0/6 | – | 1/6 | 16.2 | |
| 6.8 | 0/6 | – | 0/6 | – | |
| NP | 6400 | 6/6 | 18.6 ± 0.2 | 6/6 | 11.7 ± 1.4 |
| 640 | 6/6 | 21.7 ± 0.8 | 6/6 | 12.3 ± 1.0 | |
| 64 | 5/6 | 32.6 ± 5.6 | 3/6 | 14.0 ± 1.1 | |
| 6.4 | 1/6 | 30.9 | 0/6 | – | |
| 0.64 | 0/6 | – | 0/6 | – | |
aTenfold serially diluted synthesized RNA with partial genome sequences of COD/76/Mayinga strains was used in this experiment.
bThe number of positive results per 6 reactions with diluted RNA templates.
Tp, Time for detecting positive results (mean ± standard deviation).
–, negative results.