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. Author manuscript; available in PMC: 2016 Feb 23.
Published in final edited form as: Mol Cell. 2014 Dec 24;57(1):191–201. doi: 10.1016/j.molcel.2014.11.021

Figure 5. Multiangle light scattering (MALS) experiment demonstrating dimerization state of KIT D4D5 fragments (WT and oncogenic mutants).

Figure 5

Different concentrations of KIT D4D5 fragments were injected on to HPLC system equipped with light scattering (LS) detector and differential refractometer (RI). Weight-average molar mass was calculated based on LS and RI signals. The weight average molar masses were plotted as a function of concentration (dots on the graph). Solid line represents simulation of dimerization curves (top left panel) or nonlinear regression fit of the data to the model (Equation 1). Dimerization constants calculated by nonlinear regression fit are presented in the table (right panel).