Table 1.
Islet size | Total β-cell area | Total α-cell area | Relative β-cell area | Relative α-cell area | β-Cell proliferation index | |
---|---|---|---|---|---|---|
P2 | ||||||
Control (n = 3) | 8.2 ± 0.4 | 6.0 ± 0.2 | 2.0 ± 0.2 | 74.0 ± 2.0 | 25.0 ± 3.5 | 32.0 ± 0.2 |
Prox1endOE (n = 5) | 7.2 ± 0.7 | 4.9 ± 0.5 | 2.7 ± 0.4 | 69.0 ± 1.6 | 38.2 ± 2.5 | 14.1 ± 2.8 |
P value | 0.33 | 0.12 | 0.09 | 0.09 | 0.02 | 0 |
P7 | ||||||
Control (n = 3) | 9.6 ± 1.1 | 8.0 ± 0.8 | 1.7 ± 0.3 | 82.6 ± 2.2 | 18.6 ± 3.0 | 9.2 ± 0.6 |
Prox1endOE (n = 6) | 6.3 ± 0.7 | 4.8 ± 0.6 | 1.9 ± 0.1 | 73.5 ± 2.0 | 32.9 ± 3.4 | 3.4 ± 0.5 |
P value | 0.03 | 0.02 | 0.5 | 0.03 | 0.03 | 0.001 |
Islet size = synaptophysin+ area × 103 U area. Total (β/α) cell area = (insulin/glucagon)+ area × 103 U area. Relative (β/α) cell area = (insulin/glucagon)+ area/total islet area × 103 U area. β-Cell proliferation index = number of Ki67+ cells/insulin+ area. U are arbitrary units as determined by the AxioVision software. Numbers in bold indicate significant P values. Results are shown as mean ± SEM.