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. 2016 Jan 5;25(6):1100–1115. doi: 10.1093/hmg/ddv633

Figure 4.

Figure 4.

Effects of CHMP2B on cell-to-cell transmission of α-syn when added to donor cells. (A) The in vitro co-culture system was used with donor neuronal cell line B103 (top) infected with both LV-control or LV-α-syn and LV-CHMP2B or LV-shCHMP2B. (B) Uninfected acceptor neuronal cell line B103 (bottom) were examined by immunohistochemistry for MAP2 (green), α-syn (red) and nuclei (DAPI, blue). Panels are presented as merged and split image for α-syn (red). (C) Neuronal cells were analyzed with ImageJ to determine levels of α-syn immunoreactivity expressed as pixel intensity. (D) Supernatant from the acceptor cell chamber was assayed by electrochemical assay for α-syn. *Indicates statistical significance P < 0.05 compared with acceptor cells with LV-control only. #Statistical significance P < 0.05 compared with acceptor cells with LV-α-syn alone. One-way ANOVA with post hoc Tukey–Krammer (n = 3 experiments per condition).