Table 1. Protein antigens recognized by IgG antibodies in asymptomatic dog (AD) sera.
| Function | IDa | Protein Name | TriTrypDB accession no. L. infantum | Mrb (pred/exp) | pIc (pred/exp) | Pm/% Scd | Scoree |
|---|---|---|---|---|---|---|---|
| Carbohydrate metabolism | 1 | Dihydrolipoamide dehydrogenase | LinJ.32.3510 | 50.6/54.75 | 6.87/6.06 | 16/51.68 | 555.79 |
| 7 | Aldose-1-epimerase | LinJ.35.0990 | 41.5/43.41 | 6.24/6.48 | 10/46.90 | 168.41 | |
| 10 | Prostaglandin f2-alpha synthase | LinJ.31.2210 | 31.7/31.9 | 6.65/6.87 | 12/65.49 | 385.72 | |
| 12 | Phosphomannomutase | LinJ.36.2070 | 28.1/27.7 | 5.57/5.64 | 7/44.13 | 109.50 | |
| 17 | Enolase | LinJ.14.1240 | 46.0/49.58 | 5.45/5.93 | 17/62.00 | 331.13 | |
| Amino acid metabolism | 11 | 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase | LinJ.25.2090 | 30.4/29.5 | 6.21/6.30 | 9/39.07 | 78.87 |
| 15 | Pyrroline-5-carboxylase reductase | LinJ.13.1420 | 28.3/27.7 | 6.57/6.59 | 2/11.4 | 54.97 | |
| Transcription/Translation | 2 | RNA helicase | LinJ.21.1820 | 58.8/47.50 | 8.62/6.16 | 22/53.97 | 294.73 |
| 2 | Eukaryotic initiation factor 4a | LinJ.01.0790 | 45.3/47.50 | 6.23/6.16 | 13/51.12 | 79.8 | |
| 4 | Proteasome beta 2 subunit | LinJ.35.3880 | 27.6/25.62 | 6.61/6.34 | 8/29.92 | 158.9 | |
| 9 | RNA-binding protein | LinJ.35.2240 | 30.2/32.5 | 8.13/6.74 | 9/67.88 | 155.85 | |
| Protein targeting/Signal tranduction | 3 | GTP-binding protein | LinJ.25.1460 | 24.2/26.25 | 6.54/6.27 | 9/44.44 | 197.25 |
| 6 | Cyclophilin 2 | LinJ.06.0120 | 20.4/19.60 | 7.03/6.48 | 7/68.98 | 165.29 | |
| 8 | Cyclophilin 40 | LinJ.35.4830 | 38.5/41.36 | 6.00/6.52 | 10/42.37 | 128.70 | |
| 9 | Activated protein kinase c receptor (LACK) | LinJ.28.2940 | 34.4/32.5 | 6.52/6.74 | 6/31.73 | 110.41 | |
| 16 | Chaperonin HSP60, mitochondrial precursor | LinJ.32.1940 | 64.3/62.0 | 6.49/6.66 | 12/36.53 | 114.47 | |
| Uncharacterized proteins | 5 | Ribonucleoprotein p18, mitochondrial precursor | LinJ.15.0330 | 21.3/21.91 | 7.15/6.10 | 2/67.91 | 345.04 |
| 8 | Protein transport protein Sec13 | LinJ.32.0050 | 36.3/41.36 | 6.02/6.52 | 6/33.63 | 52.83 | |
| 13 | i/6 autoantigen-like protein | LinJ.22.1310 | 23.0/27.7 | 5.83/5.73 | 6/35.27 | 35.66 | |
| Hypothetical proteins | 13 | Hypothetical protein, conserved | LinJ.09.0040 | 22.3/27.7 | 5.87/5.73 | 5/50.48 | 25.02 |
| 14 | Hypothetical protein, conserved | LinJ.19.1440 | 25.9/28.3 | 6.35/6.54 | 10/53.51 | 110.44 |
aID—The numbers correspond to the specific spots indicated in Fig 3.
bMr—Molecular weight, kDa (pred, predicted; exp, experimental).
cpI—Isoelectric point.
dPm—No. of peptides matched / % Sc (sequence coverage percentage).
eScore—The sum of all peptide Xcorr values above the specified score threshold (+2 ≥ 2.0, +3 ≥2.5).