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. 2016 Feb 23;11(2):e0150102. doi: 10.1371/journal.pone.0150102

Fig 8. In vitro studies on primary rat osteoblasts and osteoclasts.

Fig 8

A. Alkaline phosphatase staining of primary osteoblasts isolated from 5HT sublines. B. TRAP staining of primary osteoclasts isolated from 5HT sublines. C-D. Expression of mRNA for osteoblast (Alph, Ocn) and osteoclast (Trap, Ctsk) differentiation markers and 5HT-related molecules (5HTT, Tph1, 5HT-1B, -2A and -2B receptors) in rat primary osteoblast (C) and osteoclast cultures (D) (n = 3–5). E. Levels of 5HT measured in media from primary high-5HT and low-5HT rat osteoblasts and osteoclasts (n = 4). F. Effects of added 5HT, insulin and their combination on Alph and Ocn expression from primary osteoblasts isolated from high-5HT and low-5HT rats (n = 3). G-H. Effects of added 5HT, insulin and LX1032 on Ctsk, Trap, and Tph1 mRNA expression (G) (n = 3) and osteoclast number (H) (n = 4–7 wells analyzed per group) in primary osteoclast cultures from control Wistar rats. Expression data (C, D, F, G) are shown as fold difference over a control (indicated by the dashed line) with 95% confidence intervals. Stars (*) at estimates indicate significance vs. control, whereas horizontal lines and associated stars indicate significance in fold difference over control between treatments.