Fig 1. Characterization of FGFR1 amplification, expression and sensitivity to AZD4547 in lung cancer cell lines.

A) FGFR1 copy number was determined in a lung cell line panel by qPCR. Three different assays targeting 5’, 3’ and middle regions of the gene are shown. Bars: stdev; Table reflects aCGH FGFR1 copy number for each cell line. B) Sensitivity to FGFR inhibition by AZD4547 treatment was determined by MTS proliferation assay. Sensitive cells lines were defined by GI₅₀<200nM. C) Sensitivity to FGFR inhibition by AZD4547 was determined in a long term clonogenic assay in cell lines indicated. Mean colony count was normalised to DMSO control for each cell line. D) Expression of FGFR1 mRNA was determined in AZD4547 sensitive and resistant cell lines by nanoString analysis. E) Expression of FGFR1 protein was determined by western blot in indicated cell lines.