Fig 1. Wild yeast strains from different origins accumulate higher levels of triacylglycerol (TAG) and exhibit longer chronological lifespan.

(A) Growth curves of wild strains isolated from oak exudates, vineyards, and clinical samples vs. three laboratory strains. Cells were grown in YPD at 30° in a 96-well plate. Each curve represents the average of growth of 2 to 4 strains in the category. Shown are representative results from two biological replicates. (B) Chronological lifespan in SC medium was measured by the “outgrowth” approach. (C) Chronological lifespan examined by spot assays. Viability of cultures was assayed every 3 to 5 days for two months. Only day 1 (all strains showed close to 100% viability) and day 61 images are shown. Cultures were 10-fold serially diluted in water before inoculation. (D) Quantification of triacylglycerol of cells harvested from day 1 and day 8 post-saturation YPD cultures.