Table 2. Insertion location, integration site in soybean genome and 5’ and 3’ Flanking Junction reads by Target genome sequencing of TE1 and TE2 single and TE1 x TE2 breeding stack events.
| Trait | Breeding Generation and Sample Number | Integration site in soybean genome | Reads mapped to transgene (Avg. coverage) | Number of Junction Pair End (PE) pairs | 5’ Junction Reads coverage | 3’ Junction Reads coverage |
|---|---|---|---|---|---|---|
| TE 1 | F2-1 | Gm06:18,552,249..18,556,606 | 279797 (6053x) | 50970 | 4905 | 7013 |
| TE 1 | T3-3 | Gm06:18,552,249..18,556,606 | 267750 (5792x) | 49607 | 4692 | 6919 |
| TE 2 | F2-10 | Gm02:10,027,285..10,027,342 | 161770 (2821x) | 17722 | 1847 | 4029 |
| TE 2 | T3-1 | Gm02:10,027,285..10,027,342 | 265192 (4625x) | 28051 | 3001 | 6006 |
| TE 1 in TE 1 x TE2 stack | Single generation | Gm06:18,552,249..18,556,606 | 362253 (7837x) | 62534 | 9206 | 10587 |
| TE 2 in TE 1 x TE 2 stack | Single generation | Gm02:10,027,285..10,027,342 | 455842 (7950x) | 25019 | 6987 | 18792 |