Table 4. Molecular characterization of soybean single events, TE 1 and TE2, and their breeding stack TE1× TE2 using target capture sequencing approaches.

Each element of the T-DNA is represented by the “X” amount of coverage depth. Both T-DNA inserts within TE1 and TE2 events share several identical elements, such as T1_E5 = T2_E1 (promoters), T1_E7 = T2_E3 = T2_E6 (terminators), T1_E8 = T2_E4 = T2_E7 (promoters), T1_E9 = T2_E8 (gene of interest, GOI), and T1_E10 = T2_E9 (terminators).

TE1			TE1 in TE1 × TE2
Generations	F2_1	T3_3	Single generation
Elements	Coverage (nX)
T1_E1-expression enhancer	14874	15877	14871
T1_E2 –terminator	7433	7244	6711
T1_E3 –GOI	189	131	78
T1_E4 –promoter	2126	1743	1653
T1_E5 –promoter	2392	1883	1774
T1_E6 –GOI	5171	4476	4242
T1_E7 –terminator	12914	11770	11373
T1_E8 –promoter	15291	14219	13808
T1_E9 –GOI	12804	11951	11788
T1_E10—terminator	11033	9007	8800
TE2			TE2 in TE1 × TE2
Generations	F2_10	T3-1	Single Generation
Elements	Coverage (nX)
T2_E1 –promoter	1881	3049	4869
T2_E2 –GOI	2409	3738	5401
T2_E3 –terminator	5294	9086	13035
T2_E4 –promoter	5907	10313	14303
T2_E5 –GOI	1426	2358	3653
T2_E6 –terminator	5182	8776	12930
T2_E7 –promoter	6799	11757	17953
T2_E8 –GOI	5905	9692	16089
T2_E9—terminator	3984	6342	13483