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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Br J Haematol. 2015 Dec 18;172(5):794–806. doi: 10.1111/bjh.13862

Figure 1. Resveratrol improves platelet spreading after storage.

Figure 1

Human platelets were collected from healthy human donors and platelet concentrates (PC) were prepared according to standard blood banking procedures. PC were treated with vehicle or 10 μM resveratrol (Resv.) prior to storage and stored for up to 5 days at room temperature with agitation. After 1, 3 or 5 days of storage, platelets were washed and allowed to spread on fibrinogen-coated coverslips for 45 min. Coverslips were fixed and visualized by differential interference contrast (DIC) optics using an Olympus BX51 microscope at 100X and SPOT computer software. Scale bars = 10 μm. One representative donor of 3 is shown (A–B). The percentage of fully spread platelets per field of view was determined by manually counting four fields of view for 5 individual donors (C). Mean +/− SEM. n=5 Statistical significance determined by Two-Way RM ANOVA with Dunnett’s post-test. † p<0.05, †† p<0.01 compared to Day 1. *p<0.05, **p<0.01 compared to vehicle.