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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Hepatology. 2015 Dec 11;63(3):731–744. doi: 10.1002/hep.28252

Figure 5. Mlk3−/− mice are protected against high fat, fructose and cholesterol (FFC) diet-induced liver injury and inflammation and have reduced plasma level of CXCL10 enriched extracellular vesicles (EVs).

Figure 5

Wild type (WT) & mixed lineage kinase (Mlk3)−/− mice were fed either chow or FFC diet. (A) Plasma alanine aminotransferase (ALT) levels were measured. Total RNA was extracted from liver tissue and mRNA expression of (B) surface macrophage markers cluster of differentiation (CD) 68 and F4/80, (C) macrophage cytokines tumor necrosis factor (TNF) α, and interleukin (IL) 6, and (E) CXCL10 expression were evaluated by real-time qPCR. (D) Enzyme linked immunosorbant assay (ELISA) for (C-X-C motif) ligand 10 (CXCL10) was performed on (E) the whole plasma and EVs isolated from the plasma of FFC and chow fed WT and Mlk3−/− mice. Bar columns represent mean ± S.E.M. *** P < .001, ** P < .01, * P < .05 compared to WT chow-fed mice