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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Biochim Biophys Acta. 2016 Jan 14;1860(4):678–685. doi: 10.1016/j.bbagen.2016.01.006

Figure 5. Effect of Resveratrol on FcεRI-induced phosphorylation of Syk in human skin mast cells.

Figure 5

Phosphorylation of Syk (Y525) was determined by quantitative infrared Western blotting of whole cell lysates of sensitized mast cells pre-treated with 1, 10 or 100 µM Resveratrol or DMSO (vehicle), and activated for 5 min with 100 ng/ml NP-BSA. The blot shown (a) is representative of 3 independent experiments with mast cells from skin tissue of different donors. Percent phosphorylation expressed as mean ± SEM (b, n=3) was determined as the ratio of phosphorylated Syk (Y525) to total Syk using fluorescent signal values obtained with an infrared imager. Statistical significance was determined by student’s t-test comparing to values from mast cells activated without Resveratrol pre-treatment. *, p<0.05.