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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: J Mol Cell Cardiol. 2016 Jan 9;91:160–171. doi: 10.1016/j.yjmcc.2016.01.003

Fig. 5. 5-Aza-2’-deoxycytidine reversed antenatal hypoxia-induced GR promoter hypermethylation and gene repression in offspring hearts.

Fig. 5

Pregnant rats were treated with normoxia control or hypoxia at 10.5% O2 from day 15 to day 21 of gestation. Newborn rats were injected with saline (−AZA) or 5-aza-2’-deoxycytidine (+AZA) (1 μg/g/day, i.p.) at postnatal day 1 and day 3. At 4 week old, rats were sacrificed and hearts were isolated. Global DNA methylation levels were measured using a 5-mC DNA ELISA kit (A). DNA methylation at GR promoter 14, 15, 16 and 17 was determined by MeDIP (B). mRNA abundance of GR exon 14, 15, 16, 17 (C) and total GR mRNA (D) was measured using real-time RT-PCR. GR protein abundance was measured with Western blot (E). Data are mean ± SEM, n = 4-6. * P < 0.05, hypoxia vs. control, † P < 0.05, control 4 week offspring vs. control fetus.