(A) Schematic diagram illustrating the general direction of PCP protein polarity (arrows), expression gradient of Ds (magenta) and organization of Ds-Fat and Fz PCP pathway components in the Drosophila wing disc. (B) Western blots, using antibodies indicated on the right, showing the results of co-immunoprecipitation experiments between V5-tagged Dachs (lanes 1–3,7), Ds-ICD (lanes 4–6,8) or GFP (lanes 9–11) of Flag-tagged Sple (lanes 1,4,9), Sple-N (lanes 2,5,10), Pk (lanes 3,6,11) or GFP (lanes 7,8). Upper panels (Input) show blots on lysates of S2 cells, lower panels (IP-V5) show blots on proteins precipitated from these lysates by anti-V5 beads. Similar results were obtained in three independent biological replicates of this experiment. (C–G) Portions of wing imaginal discs with clones of cells expressing GFP:Sple (C,E,F) or GFP:Pk (D,G) (green), stained for expression of E-cadherin (blue), and showing either anti-Wg (C–E) or hh-Gal4 UAS-mCD8-RFP (F,G) (red). White arrows indicate direction of polarization of Sple or Pk.
DOI:
http://dx.doi.org/10.7554/eLife.09946.003