(A–D) Hair polarity in pleura revealed by F-actin (phalloidin staining) in wild type (A), and in pk-sple14 (B), pk30 (C) and sple1 (D) mutants. Yellow asterisk indicates the position of the spiracle, which forms near the center of the anterior compartment. Yellow arrows indicate the region where hair orientation is normal, and red arrows indicate the region where hair orientation is disrupted. Dashed yellow lines mark approximate boundaries between regions with normal and abnormal polarity. (E–H) Hair polarity in tergites of wild-type (E), pk-sple14 (F), pk30 (G) and sple1 (H) mutant animals. Black arrows indicate regions where hair orientation is normal, and blue arrows indicate the region where hair orientation is abnormal. Dashed blue line mark approximate boundaries between regions with normal and abnormal polarity.
DOI:
http://dx.doi.org/10.7554/eLife.09946.015