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Shown are immunoblots (IB) of Itpkb immunoprecipitates (IP, top) or whole cell lysates (WCL, bottom) from Itpkb+/+ or Itpkb-/- DN3 cell-enriched Rag2-/- thymocytes (left) or sorted CD53- DP thymocytes (right), resolved via SDS-PAGE and probed with antibodies against Itpkb (top) or PLCγ1 (bottom, loading control) as in (Miller et al., 2007; Huang et al., 2007).
