Figure 10. Itpkb-loss reduces the Notch-dependence of DN thymocyte development to DP cells in vivo.

Shown are (A) CD4/CD8 expression on total thymocytes and (B) HSA/TCRβ expression on CD4^-CD8⁺ thymocytes from Rag2^-/- and Rag2^-/-Itpkb^-/- mice two days post α-CD3 antibody injection. Starting 3–4 hr before α-CD3 injection, the mice were treated once daily with orally administered γ-secretase inhibitor LY-411,575 or vehicle (Wong et al., 2004). Numbers indicate % cells per respective gate. The gates in (B) denote CD8⁺HSA^high ISP (Petrie and Zuniga-Pflucker, 2007; Xiong et al., 2011). Representative of two independent experiments (n = 3).

DOI: http://dx.doi.org/10.7554/eLife.10786.014