Author response image 2. Retained intron transcripts found in polysomes are also observed by ribosome profiling.
(A) An example of a retained intron transcript on polysomes where the retained intron region is detected through the eight+ ribosome fraction. (bottom) Ribosome profiling reads are detected in the retained intron region in two separate 293T ribosome profiling datasets. (B) Shown are fragment length distributions for ribosome profiling reads derived from either retained intro regions (red) or coding sequence regions (black). The FLOSS score (Ingolia et al., 2014) between retained intron and CDS fragment length distributions is 0.023, indicating near perfect agreement. The FLOSS score is the integrated point wise difference between the two distributions. Ribosome profiling data from the Yoon-Jae Cho lab and from Sidrauski et al. (2015).