Video 5. Ribosomes are visualized with photo-activated localization microscopy (PALM) in fibroblasts stably expressing the L10A 60s large subunit tagged with photo-activated TagRFP (PATagRFP).
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DOI: 10.7554/eLife.10415.013
405-nm activation energy is controlled manually to limit the number of fluorescent molecules, enabling single particle tracking of ribosomes. Focal adhesions are labeled with vinculin-GFP. Ribosomes are tracked with a streaming acquisition at 35 ms per frame. The movie is played at 30 fps with a 5 µm scale bar.