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. 2016 Feb 15;5:e11181. doi: 10.7554/eLife.11181

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© 2016, Delventhal et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 9. — All genotypes have identical 2^nd chromosomes: ΔGr59c, Gr89a-GAL4. Flies were subjected to the four indicated temperature regimes. (a) GAL80^ts parental control flies without UAS-Gr59c display mutant, elevated responses to UMB, TPH, and CAF in all regimes. (b) UAS-Gr59c parental control flies without GAL80^ts in all cases display wild-type responses to BER, LOB, and DEN. (c) Experimental flies (UAS-Gr59c/GAL80^ts) display mutant responses when kept continuously at GAL80^tspermissive temperature (25°C), indicating that GAL80^ts is suppressing UAS-Gr59c expression. However, when GAL80^ts is inactivated at any time by shifting flies to higher temperature (29°C), thus activating UAS-Gr59c, flies display wild-type responses.

DOI: http://dx.doi.org/10.7554/eLife.11181.015