Fig. 6.
A developmentally-regulated neurosteroid tone influences the decay time of mIPSCs recorded from α1−/−L2/3 neurons. A bar graph showing the effect of γ-CD pre-incubation (1 mM) on the τW of mIPSCs recorded from α1−/− L2/3 pyramidal neurons during development (n = 4–13 cells). In common with WT L2/3 pyramidal neurons, treatment of α1−/− cortical brain slices with γ-CD results in faster decaying mIPSCs at P7 - 8, P10 and P15, but not at P20-24. For comparison, the effect of finasteride (50 μM) pre-incubation at P7 - 8 and P20 - 24 is also shown (n = 7–14 cells). †† = p < 0.01, † = p < 0.05 vs control, post hoc Tukey HSD test following a two-way ANOVA. ** = p < 0.01, *** = p < 0.001, vs control, post-hoc independent samples t-test following a two-way ANOVA. n.s. = not significant. The independent variables for the two-way ANOVAs were postnatal age and treatment. Illustrated above each developmental time point is the corresponding averaged and superimposed mIPSCs, normalised with respect to peak amplitude, obtained from representative pyramidal neurons of WT and α1−/− in the absence (control, black line) and following 1 mM γ-CD pre-incubation (grey line).