Fig. 1.

Backscattered scanning electron microscopy images collected from the mid shaft (transverse cross section) of the cortical bone of the WT (A) and Crh^− 120/+ mice (B). Top images are full transverse cross section and areas surrounded by white dash lines represent cross section of mechanically tested sample. Higher magnification BSE images shown in the bottom panel are obtained from the anterior regions (white squares in top images) of the cortex. In Crh^− 120/+ bone two distinct regions were observed. Dark gray sections (i) surrounded by white bands (cement lines) were observed near cavities in Crh^− 120/+ bone and (ii) periosteal cortex (no cavities) were observed areas (50 × 50 μm) surrounded by black squares (i and ii) in bottom panel of A and B were used for quantification of mineralization parameters.