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. 2016 Feb 22;12(2):e1005886. doi: 10.1371/journal.pgen.1005886

Fig 6. Glial Csk regulates Fasciclin II localization at the NMJ.

Fig 6

(A-E) Immunostaining of anti-FasII (green), anti-Dlg (red), and anti-HRP (blue) at NMJs with the following genotypes: (A-A”) wild type, (B-B”) Cskc04256, (C-C”) Cskc04256/Cskj1D8, (D-D”) Csk-RNAi expressed in the whole animal (Tubulin-Gal4), and (E-E”) Csk-RNAi expressed only in glia (Nrv2-Gal4). Extra-synaptic FasII was defined as FasII signal found outside the Dlg-stained region. Areas with high levels of extra-synaptic FasII are indicated with white arrows. Scale bar = 10 μm for A and 5 μm for A”. (F) Relative levels of extra-synaptic FasII debris (extra-synaptic FasII staining area/total FasII staining area) present at the synapse. Values are represented as a percent of wild type to allow for appropriate comparison between multiple immunostaining experiments. For details on quantification of extra-synaptic FasII levels, see Methods. * p < 0.05, ** p < 0.01, *** p < 0.001 by Student’s T-test compared to wild type.