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. Author manuscript; available in PMC: 2016 Aug 7.
Published in final edited form as: Biochem Biophys Res Commun. 2015 May 20;463(4):483–489. doi: 10.1016/j.bbrc.2015.04.151

Figure 3.

Figure 3

Sec22 regulates Wg trafficking and is packaged together with Wg and p24.

Two mutant alleles of Sec22 were generated through P-element-mediated imprecise excision (A). Drosophila S2 cell was fed with dsRNA of Sec22, Vps35, GFP and lacZ. Wg protein in the supernatant (S) and in the cell lysate (lysate) were detected (B). Wg accumulates (C′) in the Wg-producing cells bearing Sec22 homozygous mutant clones marked by the absence of GFP (C). Baiser-V5 (D′), Sec22-HA (D″) and Wg (D‴) co-localize precisely (D‴′) and DAPI is shown in (D).