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. 2016 Feb 24;7:200. doi: 10.3389/fpls.2016.00200

FIGURE 4.

FIGURE 4

Truncated psaK 5′ UTRs provide high levels of transgene production. Leaves of N. benthamiana were agroinfiltrated with non-replicating vectors containing different psaK 5′ UTRs, or the TMV 5′ UTR, upstream from the GFP gene (pGFPe-XX, where XX denotes the 5′ UTR). Agroinfiltrated leaf tissue was harvested at 5 DPI and leaf extracts were analyzed by SDS-PAGE followed by coomassie staining. GFP band intensity was quantified using ImageJ software, using native plant protein bands as a loading control. Columns represent means ± standard error from four independently infiltrated samples. Two stars (∗∗) indicate p < 0.05 and three stars (∗∗∗) indicate p < 0.01 as compared to TMV by Student’s t-test.