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. 2016 Feb 24;7:200. doi: 10.3389/fpls.2016.00200

FIGURE 8.

FIGURE 8

BeYDV vector improvements enhance transgene production synergistically. (A) Summary of improvements in NVCP production. Extracts from leaves of N. benthamiana agroinfiltrated with BeYDV NVCP vectors were analyzed by sandwich ELISA. Data were normalized for total soluble protein. Columns represent results from four or more independently infiltrated leaf samples ± standard error. Two stars (∗∗) indicates p < 0.05 as compared to AtPsaK/Ext/EHA105 by Student’s t-test. Transgenic, estimated yield (Santi et al., 2006); TEV, tobacco etch virus 5′ UTR; VspB, 3′ UTR from soybean vspB gene; LBA4404, Agrobacterium strain LBA4404; Ext, tobacco extensin terminator; TMV, tobacco mosaic virus 5′ UTR; GV3101, Agrobacterium strain GV3101; EHA105, Agrobacterium strain EHA105; MAR, tobacco Rb7 matrix attachment region inserted 3′ of the gene terminator; psaK, 5′ UTR from A. thaliana psaK gene. (B) Extracts from leaves of N. benthamiana agroinfiltrated using strain EHA105 with BeYDV GFP (pBYR2eP3-GFPM), NVCP (pBYR2eP3-sNVM), or rituximab vectors (Rtx, pBYR2ePSI-MrtxGM + pBYR2ePSI-MrtxKM) were separated on SDS-PAGE gels and stained with Coomassie dye. The bands corresponding to GFP (26 kDa) and NVCP (58 kDa) are indicated, along with the rubisco large subunit rbcL.