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. 2016 Feb 10;2016:4245028. doi: 10.1155/2016/4245028

Figure 4.

Figure 4

In the absence of the IL-4Rα alternative macrophage activation is obliterated in Mtb-infected IL13tg mice. BALB/c (white circles), IL-13tg (black circles), and IL-13tg  × IL-4Rα −/− (black rhombs) mice were infected with 100 CFU Mtb H37Rv per aerosol. At different time points, gene expression of (a) fizz1, (b) ym1, (c) arg1, and (d) arginase activity in lung homogenates of infected mice was determined. Results represent means and standard deviations of 4-5 mice (∗ p < 0.05 for comparison of IL-13tg and BALB/c mice; # p < 0.05 for comparison of IL-13tg and IL-13tg  × IL-4Rα −/− mice; nonparametric ANOVA with Dunn's Multiple Comparison Test). One experiment representative of two performed is shown. (e) For immunohistological detection of Arg-1, lung sections were prepared from mice at 113 days after infection (n = 4-5). Arg-1 expression was not found in the lungs of Mtb-infected BALB/c mice but was prominent in granulomas of IL-13tg mice surrounding the necrotic center. Deletion of the IL-4Rα abolished Arg-1 expression in lungs of IL-13tg mice. Representative photomicrographs of one experiment out of two performed are shown.