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. 2016 Feb 24;6:21898. doi: 10.1038/srep21898

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Inline graphic — Fluorescence readings of E. coli C321.ΔA cells expressing -wt or the evolved mutants, each coexpressed with BocLysRS and corresponding GFP_UV-Asn149UAGN. The expressions were conducted either in the presence or in the absence of 5 mM Boc-Lys. Fluorescence intensity was normalized to cell growth. Each data point is the average of triplicate measurements with standard deviation.

Fluorescence readings of E. coli C321.ΔA cells expressing -wt or the evolved mutants, each coexpressed with BocLysRS and corresponding GFP_UV-Asn149UAGN. The expressions were conducted either in the presence or in the absence of 5 mM Boc-Lys. Fluorescence intensity was normalized to cell growth. Each data point is the average of triplicate measurements with standard deviation.