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. 2016 Feb 24;6:21898. doi: 10.1038/srep21898

Figure 4. Cross-decoding among UAGN codons.

Figure 4

Fluorescence readings of E. coli C321.ΔA cells expressing the GFP reporter GFPUV-Asn149UAGN, BocLysRS (in plasmid pGFPUV-UAGN-BocLysRS), and tRNA mutants (in plasmid pBK-UAGN-X; X = hit number) in the presence of 5 mM Boc-Lys. Fluorescence intensity was normalized to cell growth. Each data point is the average of triplicate measurements with standard deviation.