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. 2016 Feb 24;6:21898. doi: 10.1038/srep21898

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Fluorescence readings of E. coli C321.ΔA cells expressing the GFP reporter GFP_UV-Asn149UAGN (or GFP_UV-Asn149UAG), BocLysRS (in plasmid pGFP_UV-UAGN-BocLysRS), and tRNA mutants (in plasmid pBK-UAGN-X; X = hit number) in the presence of 5 mM Boc-Lys. Fluorescence intensity was normalized to cell growth. Each data point is the average of triplicate measurements with standard deviation.