Table 1. Evolved variants with improved UAGN decoding activity. Sequences of each variant at randomized positions are listed.

Codon	tRNA variants	Positions
		29–31	32,33	33.5	37, 38	39–41
UAGA	UAGA-wt	G G A	C U	U	A A	U C C
	UAGA-1	G G G	C U	U	A U	C C U
	UAGA-2	U G G	A U	U	A C	C U U
	UAGA-3	C G G	A U	U	A C	C U U
UAGU	UAGU-wt	G G A	C U	A	A A	U C C
	UAGU-1	U G G	C U	U	A U	C U U
	UAGU-2	A G G	C U	U	A U	C U U
	UAGU-3	U G G	A U	U	A C	C U U
UAGG	UAGG-wt	G G A	C U	C	A A	U C C
	UAGG-1	U G G	C U	U	A U	C U U
	UAGG-2	G G G	C U	U	A U	C U U
	UAGG-3	A G G	C U	U	A U	C U U