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. 2016 Feb 24;6:21919. doi: 10.1038/srep21919

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Copyright © 2016, Macmillan Publishers Limited

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Panels (a,c) represent hematoxylin and eosin-stained testis sections in 4- and 5-month-old BMs. Testis sections of 4- (b) and 5-month-old (d) BMs were stained with anti-PGP9.5 antibody. Black arrowheads indicate PGP9.5-positive germ cells. Scale bars are 20 μm in all panels. GC, germ cell; IC, interstitial cell; SC, Sertoli cell. Magnification is 400× in all panels. Gonadal somatic index (GSI) = [Gonad Weight/Total Tissue Weight] × 100 (e). (*P < 0.05).