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. 2016 Feb 24;6:21919. doi: 10.1038/srep21919

Figure 3. In vitro culture and karyotyping of cryopreserved testicular cells derived from 4-month-old BM testes after thawing.

Figure 3

Total testicular cells were thawed following cryopreservation for 3 months. These cells, which were cryopreserved in StemPro-34 and DMSO (final concentration: 10%), were cultured for 7 days in StemPro-34 medium. Panels (a–d) show bright-field images captured at day 1, 3, 5, and 7, respectively. Panels e and f indicates G-banded metaphase spread in the cultures of primary and cryopreserved testicular cells, respectively. Scale bars are 100 μm and magnification is 100× in panels (a–d).