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. 2016 Feb 24;6:21643. doi: 10.1038/srep21643

Figure 3. Quaking is induced by KLF2.

Figure 3

(a) Schematic representation of the promoter of QKI gene, demonstrating a high GC content (upper panel). Bar graph shows QKI promoter-luciferase reporter activity in HEK293T cells after transduction with lenti-KLF2 virus or lenti-mock virus. Results are presented relative to reporter activity in mock transduced cells, set as 1. Mean ± s.d. from one experiment representative of three independent experiments. *P < 0.05. (b) Quantitative RT-PCR analysis of Klf2, Nos3, Qki5, Qki6 and Qki7 mRNA isolated from HUVECs transduced with mock or KLF2 overexpressing lenti-virus. Results are presented relative to cells transduced with mock virus, set as 1. Mean ± s.e.m. of n = 4. *P < 0.05. (c) Immunoblot analysis of QKI-5, QKI-6, QKI-7, NOS3 or ACTB (loading control) in protein lysates of HUVECs transduced with mock or KLF2 overexpressing lenti-virus. Bar graph shows quantification of 3 independent experiments, mean ± s.e.m. Results are relative to static cultured cells, set as 1. *P < 0.05. (d) Immunofluorescent staining of QKI-5, QKI-6, QKI-7 or NOS3 (green) and F-actin (red, rights panels) in HUVECs cultured transduced with mock or KLF2 overexpressing lenti-virus. Scale bars 100 μm.