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. 2016 Feb 24;6:21765. doi: 10.1038/srep21765

Figure 2. Hc incorporates Cn-glycans in vivo during co-infection.

Figure 2

(a) Hc binds Cn-gly during co-infection with Cn H99. Immunofluorescence punctuate surface labelling of Hc GFP recovered from lungs of Hc GFP+Cn H99 groups with GXM-binding mAb 2D10 (red) and Uvitex2B (chitin in the cell wall) after isolation from lungs of co-infected animals. In comparison, Hc recovered from lungs of Hc GFP+Cn cap59 co-infected or monospecies (Hc GFP) infected mice are not labelled by the mAb. Scale bar = 5 μm. (b) FACS demonstrates labelling of Hc cells by GXM 2D10 mAbs upon co-infection with Cn H99 or GXM added controls (in comparison to unlabelled Hc GFP (*p = 0.013 and **p = 0.0069, respectively) and Hc yeast from co-infection with Cn cap59 (#p = 0.037 and #p = 0.019).