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. 2016 Feb 22;7:10761. doi: 10.1038/ncomms10761

Figure 2. Reduced colon inflammation and production of cytokines and chemokines in DSS- treated REGγ−/− mice.

Figure 2

(a) Colon tissues from 7 days post-DSS mice were evaluated for infiltration of dendritic cells, macrophages and PMN by immunohistochemical staining with specific markers. Images are from one representative experiment of three repeats. Scale bars, 100 μM. (b) Colonic lamina propria mononuclear cells, MLN cells and splenocytes were analysed by flow cytometry after staining for CD4, CD11b, CD11c and Gr-1. Total numbers of CD4+, CD11b+, CD11c+ and Gr-1+ cells from day 7 lesions were calculated. n=6 per group. Data represent means±s.e.m. *P<0.05; **P<0.01, Student's t-test. (c) Colonic tissue explants were harvested at experimental day 7, cultured ex vivo for 24 h. Secreted cytokines were assessed from supernatants by BioPlex Multiplex and ELISA. n=10 per group. Data represent means±s.e.m. from three independent experiments. *P<0.05; **P<0.01; ***P<0.001, Student's t-test. (d) Colon epithelial cell were isolated at day 7, total RNA was extracted for expression analysis of related chemokines and cytokines by real-time RT–PCR. n=5 per group. Data represent means±s.e.m. *P<0.05; **P<0.01; ***P<0.001, Student's t-test.