Skip to main content
. 2016 Feb 22;7:10761. doi: 10.1038/ncomms10761

Figure 6. Mice with double deletion of REGγ and IκBɛ exhibit severe colitis phenotypes.

Figure 6

(a,b) Body weight (a) and Composited DAI scores (b) were recorded daily. n=10, WT and double-KO group; n=9, REGγ−/− group; and n=7, IκBɛ−/− group. Data represent means±s.e.m. from three independent experiments. *P<0.05, Student's t-test. (c,d) Mice were killed on day 7 after DSS administration. Representative colon length was shown in c and quantitated results for colon length in each group of mice were displayed (d). n=6 per group. Data represent means±s.e.m. from three independent experiments. *P<0.05; **P<0.01, Student's t-test. (e) Histopathological changes were examined by H&E staining. Representative images of distal colon section were from three repeats. Scale bars, 200 μM. (f) Colon crypt damage, ulceration and inflammation were scored individually, and composite HAI was scored. n=3 per group. Data represent means±s.e.m. of one representative experiment from three repeats. *P<0.05, Student's t-test. (g) Restoration of NFκB activity in double-KO colon epithelial cells. Each lane represents a sample from an individual mouse.