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. 2016 Feb 24;6:21925. doi: 10.1038/srep21925

Figure 1. In vitro generation of purified membrane protein antigen.

Figure 1

(a) Schematic of the IVT-HMB method for expression and purification of membrane proteins. (b) Generation of full-length proteins for 15 targets in the form of fusions to green fluorescent protein (GFP). (c) Generation of partial-length proteins for F. tularensis FTT0759 (305 amino acids) and ASFV CD2v (360 amino acids) in the form of fusions to thioredoxin. Shown are SDS-PAGE visualized by Coomassie stain (left panel of b) or silver stain (remaining panels in b,c) and containing, per lane, magnetic-bead purified fractions from 10 μL of IVT-HMB. Molecular weight (MW) in kDa is indicated, and arrowheads indicate the predicted migration position. BSA, bovine serum albumin.