Figure 5. New neurons at three microphotographs of the same field, in the nidopallium caudolateral (NCL; upper row) of turtle dove (Streptopelia turtur) and the hippocampus (HC; lower row) of reed warbler (Acrocephalus scirpaceus).
BrdU-labeled cells were identified with a rhodamine filter (a) and Hu-labeled neurons were identified with a FITC filter (b). Double-labeled neurons were identified by alternating between these two filters and by using a dual FITC-rhodamine filter to show co-localization of the two markers (c).