Table 2. Achromobacter phage phiAxp-1 gene annotations.
| Orfs | Nucleotide position | Strand | Length (aa)a | Mass (Da) | pI | Best match (aa identity) | GenBank accession number | E value | Conserved Protein Domain Family | Function |
|---|---|---|---|---|---|---|---|---|---|---|
| orf01 | 9–266 | − | 85 | 9653 | 7.80 | |||||
| orf02 | 250–1911 | − | 553 | 61431 | 8.50 | IME_AB3 gp17 (39%) | NC_023590 | 1e–128 | cd00046; pfam00270; cd00079; smart00490; COG1061; smart00487; PRK13766 | DNA/RNA helicase protein |
| orf03 | 1979–2659 | − | 226 | 23634 | 6.76 | IME_AB3 gp15 (27%) | NC_023590 | 2e–09 | ssDNA-binding protein | |
| orf04 | 2726–3406 | − | 226 | 25248 | 8.68 | KL1 orf33 (50%) | NC_018278 | 7e–67 | recombinase | |
| orf05 | 3467–3910 | − | 147 | 16336 | 7.30 | IME_AB3 gp13 (32%) | NC_023590 | 2e–15 | ||
| orf06 | 3954–4496 | − | 180 | 19897 | 5.44 | IME_AB3 gp12 (37%) | NC_023590 | 2e–17 | cd11541; COG1694; pfam03819 | MazG pyrophosphatase |
| orf07 | 4623–4838 | + | 71 | 8670 | 9.25 | IME_AB3 gp10 (33%) | NC_023590 | 2e–04 | ||
| orf08 | 4822–7245 | + | 807 | 89780 | 5.38 | vB_PaeS_SCH_Ab26 orf35 (41%) | NC_024381 | 0.0 | COG4983 | replicative primase/helicase |
| orf09 | 7593–8054 | + | 153 | 17483 | 8.44 | |||||
| orf10 | 8051–8779 | + | 242 | 27281 | 5.42 | |||||
| orf11 | 8845–9045 | + | ||||||||
| orf12 | 9050–9307 | + | ||||||||
| orf13 | 9304–9453 | + | ||||||||
| orf14 | 9450–9680 | + | 76 | 8690 | 4.64 | |||||
| orf15 | 9677–10063 | + | 128 | 15377 | 11.55 | |||||
| orf16 | 10160–10390 | + | 76 | 8457 | 8.90 | |||||
| orf17 | 10471–11160 | + | 229 | 26350 | 9.81 | |||||
| orf18 | 11162–11461 | + | 99 | 11685 | 9.40 | |||||
| orf19 | 11461–11901 | + | 146 | 17283 | 10.05 | |||||
| orf20 | 11911–12168 | + | 85 | 9758 | 8.42 | 73 orf44 (33%) | DQ163913 | 0.028 | ||
| orf21 | 12161–12370 | + | 69 | 7604 | 7.34 | |||||
| orf22 | 12367–12765 | + | 132 | 15384 | 6.16 | |||||
| orf23 | 12827–12946 | + | 39 | 4365 | 7.15 | |||||
| orf24 | 12947–13039 | + | 30 | 3101 | 8.77 | |||||
| orf25 | 13042–13254 | + | 70 | 8056 | 7.19 | |||||
| orf26 | 13256–13579 | + | 107 | 12158 | 9.90 | |||||
| orf27 | 13572–13781 | + | 69 | 8124 | 4.89 | CGphi29 orf59 (40%) | NC_020844 | 6e–04 | ||
| orf28 | 13785–14339 | + | 184 | 21030 | 7.89 | IME_AB3 gp52 (38%) | NC_023590 | 8e–27 | endonuclease | |
| orf29 | 14375–14695 | + | 106 | 11775 | 8.89 | |||||
| orf30 | 14710–14892 | + | 60 | 6533 | 10.39 | |||||
| orf31 | 14889–15101 | + | 70 | 8085 | 9.29 | |||||
| orf32 | 15098–15355 | + | 85 | 9657 | 10.40 | |||||
| orf33 | 15352–15807 | + | 151 | 16669 | 7.75 | TMA orf38(46%) | NC_015937 | 9e–29 | cd01286; COG2131; pfam00383; TIGR02571; PHA02588; TIGR00326; PRK10786 | deoxycytidylate deaminase |
| orf34 | 15804–16184 | + | 126 | 13761 | 5.97 | |||||
| orf35 | 16246–16920 | + | 224 | 24092 | 5.55 | IME_AB3 gp46 (35%) | NC_023590 | 1e–19 | ||
| orf36 | 17087–17476 | + | 129 | 14232 | 8.75 | IME_AB3 gp45 (24%) | NC_023590 | 5e–04 | ||
| orf37 | 17707–18246 | + | 179 | 20145 | 9.34 | phiHAP1 gp45 (51%) | NC_010342 | 2e–48 | pfam05838; pfam09374; COG3926 | |
| orf38 | 18247–18507 | + | 86 | 9810 | 10.70 | PRK12792 | ||||
| orf39 | 18681–19139 | + | 152 | 17157 | 5.35 | 73 orf5 (37%) | DQ163913 | 1e–15 | ||
| orf40 | 19117–20703 | + | 528 | 59917 | 5.90 | KL1 orf7 (70%) | NC_018278 | 0.0 | TIGR01547; pfam03237 | Terminase large subunit |
| orf41 | 20718–22319 | + | 533 | 58562 | 4.77 | vB_PaeKakheti25 orf8 (48%) | NC_017864 | 5e–151 | pfam13264 | Portal protein |
| orf42 | 22316–23350 | + | 344 | 38647 | 8.96 | KL1 orf9 (28%) | NC_018278 | 6e–37 | TIGR01641; pfam04233 | Head morphogenesis protein |
| orf43 | 23373–24101 | + | 242 | 26463 | 5.57 | vB_PaeS_SCH_Ab26 orf9 (46%) | NC_024381 | 2e-44 | pfam14817; TIGR03345 | Scaffold protein |
| orf44 | 24156–25133 | + | 325 | 35813 | 5.30 | IME_AB3 gp37 (54%) | NC_023590 | 1e–115 | Major capsid protein | |
| orf45 | 25187–25555 | + | 122 | 13033 | 6.33 | 73 orf11 (34%) | DQ163913 | 3e–04 | ||
| orf46 | 25623–26144 | + | 173 | 18469 | 4.73 | vB_PaeS_SCH_Ab26 orf13 (38%) | NC_024381 | 3e–21 | Virion protein | |
| orf47 | 26141–26539 | + | 132 | 14412 | 8.76 | |||||
| orf48 | 26547–26999 | + | 150 | 17006 | 8.44 | KL1 orf16 (37%) | NC_018278 | 6e–16 | Minor tail protein | |
| orf49 | 27010–28644 | + | 544 | 58390 | 4.52 | vB_PaeKakheti25 orf17 (46%) | NC_017864 | 1e–128 | Major tail tube protein | |
| orf50 | 28731–29171 | + | 146 | 15808 | 5.67 | IME_AB3 gp31 (36%) | NC_023590 | 2e–24 | Tail chaperonin | |
| orf51 | 29216–29494 | + | 92 | 10497 | 6.54 | vB_PaeS_SCH_Ab26 orf18 (40%) | NC_024381 | 5e–13 | Tail chaperonin | |
| orf52 | 29491–29934 | + | 147 | 16064 | 9.17 | 73 orf19 (55%) | DQ163913 | 8e–42 | ||
| orf53 | 29939–33073 | + | 1044 | 110963 | 6.39 | KL1 orf21 (41%) | NC_018278 | 3e–142 | TIGR02675; pfam09718; TIGR01541; COG5281 | Tail tape measure |
| orf54 | 33078–34382 | + | 434 | 49282 | 4.46 | JWX orf22(35%) | KP202969 | 5e–40 | ||
| orf55 | 34387–34839 | + | 150 | 15660 | 9.04 | 8324 orf21 (43%) | KP202970 | 7e–28 | ||
| orf56 | 34870–35967 | + | 365 | 36841 | 7.31 | |||||
| orf57 | 36005–36544 | + | 179 | 20360 | 5.47 | JWX orf25(47%) | KP202969 | 1e–46 | pfam08875 | |
| orf58 | 36541–36936 | + | 131 | 14701 | 6.32 | JWX orf26(50%) | KP202969 | 3e–38 | ||
| orf59 | 36933–40928 | + | 1331 | 145652 | 5.78 | JWX orf27(42%) | KP202969 | 0.0 | pfam09327; pfam13550; COG4733 | tail component protein |
| orf60 | 40925–41347 | + | 140 | 15403 | 4.62 | |||||
| orf61 | 41325–41516 | + | 63 | 7304 | 9.43 | TIGR03798 | ||||
| orf62 | 41560–43680 | − | 706 | 80634 | 7.12 | IME_AB3 gp20 (55%) | NC_023590 | 0.0 | cd05538; COG0417; TIGR00592; smart00486; PRK05761; pfam10108 | DNA polymerase subunit |
| orf63 | 43680–44720 | − | 346 | 36538 | 7.24 | vB_PmaS_IMEP1 gp03 (33%) | NC_026608 | 9e–53 | DNA polymerase III beta subunit | |
| orf64 | 44704–45045 | − | 100 | 11269 | 8.51 | vB_PaeKakheti25 orf29 (48%) | NC_017864 | 9e–07 |
aamino acids.