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. 2016 Feb 24;6:21986. doi: 10.1038/srep21986

Figure 5. Danshensu reduces IR-induced inflammation mediated by NF-κB.

Figure 5

(a) The transcriptional upregulation of NF-κB-regulated proinflammatory genes by IR was inhibited by treatement with danshensu, selegiline, MAOB siRNA, or PDTC. *p < 0.05 vs. non-irradiated cells; **p < 0.05 vs. irradiated cells. (b) Cellular CXCR4, IL-1β, and IL-6 expressions after IR treatment were assessed by Western blotting. Danshensu, selegiline, MAOB siRNA, and PDTC reduced their expressions after IR treatment. Coomassie blue staining was used to confirm equal protein loading. (c) The effects of MAOB and NF-κB inhibition on the secretions of IL-1β and IL-6 by irradiated NSCLC cells were evaluated by ELISA. *p < 0.05 vs. non-irradiated cells; **p < 0.05 vs. irradiated cells. (d) p65 recruitments to the promoter regions of CXCR4, IL1B, and IL6 were confirmed using a ChIP assay.