Figure 4. Confocal phosphorescence images and their images overlaid with bright-field images of living HeLa cells incubated with 500 nM of Ir1-Ir5 in DMEM with 10% FBS (pH = 7.4) for 8 min at 37 °C followed by 100 nM of MTR.

Lane 1, confocal phosphorescence images of Ir1-Ir5; Lane 2, confocal phosphorescence images of MTR; Lane 3, Bright field; Lane 4, overlay of lane 1, lane 2 and lane 3; Lane 4, the overlap coefficient of columns lane 1 and lane 2, and Pearson’s co-localization coefficients are also presented. Excitation wavelength: 405 nm (for all Ir(III) complexes), 488 nm (for MTG); emission filter: 590 ± 30 nm (for all Ir(III) complexes ) and 520 ± 20 nm (for MTG).