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. 2016 Feb 24;6:22039. doi: 10.1038/srep22039

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(a) Time-dependent confocal imaging of Ir1/MTG co-stained HeLa cells. Confocal images of HeLa cells stained with Ir1 and MTG before and after verified scans of light irradiation. Time interval per scan: 15 s. Scale bar: 20 mm (λ_ex = 405 nm, λ_em = 590 ± 30 nm). (b) Quantitative photobleaching results indicate that Ir1–Ir5 exhibited robust emission intensity under continuous light irradiation.