Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Feb 24;6:22113. doi: 10.1038/srep22113

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(A) Q-PCR analysis of AXIN2 in unstimulated or Wnt3a-stimulated (0.1 μM, 24 hrs) C3H10T1/2^pLIVc (pLIVc) and C3H10T1/2^SS18-SSX1-V5 (SS-V5) cells depleted of or over-expressing individual TCF members as indicated. Depletion was achieved using a pool of specific or control siRNA (AllStars). (B) (first 3 columns): Depletion of each TCF/LEF (as indicated) was verified in each cell population by Q-PCR. B (right columns): Over-expression of TCF4 was measured by Q –PCR. (C) TCF4 depletion was verified by Western blot analysis using an anti-TCF4 antibody and anti-βactin as loading control; (D) LEF1 depletion was verified by immunofluorescence microscopy using an anti-LEF1 antibody. (E) Over-expression of HA-tagged LEF1 was assessed by Western blot analysis using an anti-HA antibody and anti-tubulin antibody as loading control.