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. Author manuscript; available in PMC: 2016 Mar 9.
Published in final edited form as: Cell Rep. 2016 Jan 21;14(5):1142–1155. doi: 10.1016/j.celrep.2015.12.100

Figure 3. H2A.Z.1ub is necessary for maintenance of bivalent chromatin.

Figure 3

A. Box plots represent the log2 fold change in expression of either H2A.Z.1K3R3 relative to H2A.Z.1WT mESCs at RNAPII-bound genes, split into quartiles of RNAPII traveling ratios (~2459 genes/quartile) from (Rahl et al., 2010), as well as at active (11010 genes) and bivalent (1611) genes. Center lines represent the median value; box limits indicate the 25th and 75th percentiles; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, outliers are represented by dots. B–E. ChIPs for the PRC2 component, Suz12 (B), H3K27me3 (C), PRC1 component Ring1b (D), H3K4me3 (E), were assayed by qPCR. Error bars represent the SD of triplicate reactions. See also Figure S4.