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. 2016 Feb 24;17:133. doi: 10.1186/s12864-016-2456-1

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© Neuhaus et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig 1 — Four examples of new EHEC protein-coding ORFs (red arrows) discovered by ribosomal footprinting and visualized using Artemis [30]. Protein-coding ORFs are indicated by cyan arrows in the lower part of each panel. Blue lines in the upper part of each panel represent ribosomal footprint reads. a X018 is an example for a single (monocistronic) gene. b X001 is located in the upstream part of yaeO. These two genes might form a translationally coupled operon. c Two short genes, X005 and X006 are located downstream of cstA, maybe also translationally coupled. d X002 might be part of the operon yahDEFGIJ spanning from yahD to yahJ (only partly shown). The missing gene yahH had been annotated at first but was rejected later due to its structure (see Discussion and Fig 4)