Fig. 3.

ATS treatment differentially depletes T cells from the peripheral blood in vivo. NOD mice were treated with ATS or rabbit serum and the percentage of TCR-β⁺, CD4⁺, CD8⁺ and DN T cells in the peripheral blood was examined by flow cytometry. a The flow cytometry results at day 9 after the first ATS treatment indicate the measurement of the percentage of different T cell subsets. The numbers in the left panels refer to the percentages of lymphocytes in the total PBMC, the numbers in the middle panels refer to the percentages of CD3⁺TCR-β⁺ cells in the total lymphocyte pool and the numbers in the right panels refer to the percentages of CD4⁺, CD8⁺ and DN T cells among the CD3⁺TCR-β⁺ lymphocytes. T cell subsets (based on flow cytometry analysis) from the peripheral blood were followed for 30 days after two doses of ATS. (n = 4 in each group). b The percentage of CD3⁺TCR-β⁺ cells in the total lymphocyte pool. c The percentage of CD4⁺ T cells among the CD3⁺TCR-β⁺ T cells. d The percentage of CD8⁺ T cells among the CD3⁺TCR-β⁺ T cells. e The percentage of CD4⁻CD8⁻ DN T cells among the CD3⁺TCR-β⁺ T cells